Modifying Enzymes
Sigma Aldrich Fine Chemicals Biosciences Chitinase from Trichoderma viride lyophilized powder, >=600 units/g solid | MFCD00130771 | 25UN
Chitinase from Trichoderma viride lyophilized powder, >=600 units/g solid | MFCD00130771 | 25UN
EPICYPHER INC CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows - 50 rxns
CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows - 50 rxns
New England Biolabs, Inc. NcoI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence C^CATG_G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. beta-Agarase I – 100 units
B-Agarase I digests agarose, releasing trapped DNA and producing carbohydrate molecules which can no longer gel, purifying DNA fragments from gels.
New England Biolabs, Inc. HpaII Methyltransferase – 100 units
HpaII Methyltransferase recognizes the same sequence as the MspI Methyltransferase, but modifies the internal cytosine residue (C5) of the sequence CCGG.
New England Biolabs, Inc. beta-Agarase I – 500 units
B-Agarase I digests agarose, releasing trapped DNA and producing carbohydrate molecules which can no longer gel, purifying DNA fragments from gels.
New England Biolabs, Inc. E.coli DNA Ligase – 1000 units
E. coli DNA Ligase catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands in duplex DNA with cohesive ends. It is not appreciably active on blunt-ended substrates. E. coli DNA Ligase uses NAD as a cofactor and can be heat-inactivated.
New England Biolabs, Inc. Quick Ligation™ Kit – 150 reactions
The Quick Ligation Kit enables ligation of cohesive end or blunt end DNA fragments in 5 minutes at room temperature. (25C)
New England Biolabs, Inc. Antarctic Phosphatase – 5000 units
Antarctic Phosphatase (AnP) is a heat labile alkaline phosphatase purified from a recombinant source. AnP nonspecifically catalyzes the dephosphorylation of 5' and 3' ends of DNA and RNA phosphomonoesters. Also, AnP Hydrolyses ribo-, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). AnP is useful in many molecular biology applications such as the removal of phosphorylated ends of DNA and RNA for subsequent use in cloning or end-labeling of probes. In cloning, dephosphorylation prevents religation of linearized plasmid DNA. The enzyme acts on 5' protruding, 5' recessed, and blunt ends. AnP may also be used to degrade unincorporated dNTPs in PCR reactions to prepare templates for DNA sequencing or SNP analysis. AnP is completely and irreversibly inactivated by heating at 80C for 2 minutes, thereby making removal of AnP prior to ligation or end-labeling unnecessary.
New England Biolabs, Inc. RNase I – 25000 units
Ribonuclease If (RNase If) is an RNA endonuclease which will cleave at all RNA dinucleotide bonds leaving a 5'hydroxyl and 2',3' cyclic monophosphate. It has a preference for single-stranded RNA over double-stranded RNA. RNase If is a recombinant protein fusion of RNase I (from E. coli) and maltose-binding protein. It has identical activity to RNase I.
New England Biolabs, Inc. Topoisomerase I (E. coli) – 100 units
Topoisomerase I (E. coli) catalyzes the relaxation of negatively supercoiled DNA. Topoisomerase I has also been implicated in knotting and unknotting DNA and in linking complementary rings of single-stranded DNA into double-stranded rings. The intact holoenzyme is a 97 kDa protein.
New England Biolabs, Inc. RNase If – 5000 units
Ribonuclease If (RNase If) is an RNA endonuclease which will cleave at all RNA dinucleotide bonds leaving a 5'hydroxyl and 2',3' cyclic monophosphate. It has a preference for single-stranded RNA over double-stranded RNA. RNase If is a recombinant protein fusion of RNase I (from E. coli) and maltose-binding protein. It has identical activity to RNase I.